Table 2 Primers used in construction of the gcd knockout strain of A. baylyi ADP1
Primer | Sequence (5’ → 3’) |
|---|---|
U1 | GTTTCTTCGGTACCGCTTCTCTCGAATCAACCTAAATG |
U2 | GTTTCTTCCAATTGGAGACCACCTCGAATAATTTG |
D1 | GTTTCTTCCCTAGGGCATTGCCGGATACCAAATAATC |
D2 | GTTTCTTCGGCCCCCGAGGCCCATCGGTGCATAACGCTGTAC |
C1 | GCTTCTCTCGAATCAACCTAAATG |
C2 | CATCGGTGCATAACGCTGTAC |