Fig. 7

PkaA is involved in glycolysis and controls the expression of genes required for using alternative carbon sources. a glucose uptake, b hexokinase/glucokinase activity, c glycerol levels, d pyruvate levels, e α-Ketoglutarate dehydrogenase activities and f trehalose utilization in the wild-type and ΔpkaA strains. Mycelia were grown from spores in complete media and then transferred to minimal media supplemented with glucose for 24 h or to glucose and 1 M sorbitol for 10, 30 and 60 min. g Strains were grown in complete media for 24 h and then transferred to minimal media supplemented with 1 % cellulose for 24 and 120 h before the ADP/ATP ratio was measured in mycelia cell extracts. Experiments were carried out in biological triplicates and the statistical significance of *p < 0.05, **p < 0.01 and ***p < 0.001