Table 5 List of the primer pair used in this work
From: Aspergillus nidulans protein kinase A plays an important role in cellulase production
Primer | Sequence |
|---|---|
pRS426-5′ PKA UTR F | TAACGCCAGGGTTTTCCCAGTCACGACGTTCTGAAGCCCGATACAACC |
PKA Spacer GFP R | AAAGTTCTTCTCCTTTACTCATTCCCCGTGTTCCGAAATCGGGGAACAGGTGACCG |
PyrG 3 UTR PKA F | AAGAGCATTGTTTGAGGCGAATTCACCCTCTAACGAGTGATG |
PKA 3 UTR-pRS426 R | GCGGTTAACAATTTCTCTCTGGAAACAGCTCTAAGGCAGGCAGTTCTCG |
GFP PyrG FW | GCATGCAAGCTTGGCGTATTCTGTCTGAGAGGAGGC |
Afu PyrG RV FGSC | GAGCAGCGTAGATGCCTCGACC |
Spacer GFP FW | GGAACACGGGGAATGAGTAAAGGAGAAGAACT |
GFP Ve 3 Afu RV | CTCAGACAGAATACGCCAAGCTTG |
pRS426-5′ snfA UTR F | GTAACGCCAGGGTTTTCCCAGTCACGACGTGGAGATGGAAGTCGAAAGG |
CreA GFP | ATAGACATGCCGTCACATGG |
Afu pyrG pCDS60 R | GAGCAGCGTAGATGCCTCGACC |
F_pkA_checkinsert_A | ATGGGTCCGACACCAAGA |