Fig. 2
From: Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules
SDS PAGE analysis of proteins and enzymes. a Expression of GFP_CBM constructs in the cell-free translation reaction. b GFP_CBMs after Ni-IMAC purification from the cell-free translation reaction. These samples were used in experiments of Figs. 3 and 5. c Expression profile of CelEcc_CBM constructs in the cell-free translation reaction. These samples were used in experiments of Fig. 4. Red stars indicate the position of the synthesized proteins. Molecular weights calculated from the gene sequence and estimated protein concentrations are provided in Additional file 1: Tables S1, S4