Fig. 3

Affinity gel electrophoresis characterization of GFP_CBM binding to hydroxyethyl cellulose. GFP_CBMs purified from the translation reaction using Ni-IMAC were used in these experiments. Binding was detected as a difference in migration for the “No substrate” gel compared to the hydroxyethyl cellulose gel. Red stars indicate GFP_CBM fusions assigned to have altered migration, and so are inferred to have binding properties. Images of other electrophoresis gels containing lichenan, galactomannan, beechwood xylan, and arabinoxylan are provided in Additional file 2: Figure S1. Binding assignments made from all affinity gel electrophoresis studies are summarized in Table 1. Soybean trypsin inhibitor (STI) was used as a control