Fig. 6

Domain structures/relative sizes of CelEcc_CBM hybrids and reaction with pretreated biomass. CelEcc_CBMs used in this experiment were expressed in E. coli and purified as described in “Methods”. The linker is from the CipA scaffoldin of the R. thermocellum cellulosome (amino acids 323–364 of Cthe_3077). Enzyme information is aligned with the yield of total soluble hexose and pentose products detected by quantitative NIMS analysis [61] after 24-h reaction in 50 mM phosphate, pH 6.0, at 60 °C. All reactions contained 0.32 µmol of enzyme active sites and 1 mg of ionic liquid pretreated switchgrass (IL-SG). The fractional sugar content of IL-SG (1 mg) was glucose (0.47 mg); xylose (0.18 mg), arabinose (0.03 mg), other sugars, lignin, and ash (0.32 mg). Percentage yields are based on these values and the NIMS product quantitation