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Fig. 8 | Biotechnology for Biofuels

Fig. 8

From: Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules

Fig. 8

Analysis of the time course for formation of individual hexose products. CelEcc_CBMs used in this experiment were expressed in E. coli and purified as described in “Methods”. Reactions included CelEcc_CBM hybrids with IL-SG (0.32 µmol enzyme and 1 mg biomass in a total reaction volume of 0.1 mL). Cellulose fraction in unreacted biomass (green solid circles); glucose (g1, blue diamonds); cellobiose (g2, purple down triangles); cellotriose (g3, black up triangles); cellotetrose (p4, red squares). a CelEcc. b CelEcc_CBM3a. c CelEcc_CBM6. d CelEcc_CBM22. e CelEcc_CBM30. f CelEcc_CBM44. Solid lines are the results of simulation based on the kinetic scheme in Fig. 7a and the differential equations shown in Additional file 3: Differential equations

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