Fig. 9
From: Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules
Analysis of the time course for formation of individual pentose products. CelEcc_CBMs used in this experiment were expressed in E. coli and purified as described in “Methods”. Reactions included CelEcc_CBM hybrids (0.32 µmol enzyme and 1 mg biomass in a total reaction volume of 0.1 mL) with IL-SG. Hemicellulose fraction in unreacted biomass (green solid circles); pentose (p1, blue diamonds); pentobiose (p2, purple down triangles); pentotriose (p3, black up triangles); pentotetraose (p4, red squares); pentopentaose (p5, brown open circles). a CelEcc. b CelEcc_CBM3a. c CelEcc_CBM6. d CelEcc_CBM22. e CelEcc_CBM30. f CelEcc_CBM44. Solid color lines are the results of simulation based on the kinetic scheme in Fig. 7b and the differential equations shown in Additional file 3: Differential equations