Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules

Table 4 Apparent rates obtained from numerical integration of NIMS time course data

CBM fusion^b	None	CBM3a	CBM6	CBM22	CBM30	CBM44
Protein (nmol)^c	0.32	0.32	0.32	0.32	0.32	0.32
Total hexose added (mM)^d	26.1	26.1	26.1	26.1	26.1	26.1
Fraction hexose reacted^e	0.33	0.42	0.38	0.29	0.45	0.49
Apparent rate^a
k1	0.013	0.011	0.009	0.009	0.017	0.027
k2	0.225	0.103	0.111	0.099	0.186	0.232
k3	0.049	0.024	0.025	0.024	0.035	0.028
k4	0.000	0.000	0.004	0.001	0.084	0.000
k5	0.044	0.016	0.007	0.002	0.011	0.003
k6	0.000	0.009	0.001	0.022	0.016	0.010
k7	0.000	0.003	0.000	0.008	0.000	0.000
k8	0.007	0.000	0.003	0.010	0.000	0.011
Total pentose added (mM)^d	13.9	13.9	13.9	13.9	13.9	13.9
Fraction pentose reacted^e	0.56	0.54	0.52	0.50	0.54	0.58
Apparent rate^a
k1	0.006	0.003	0.004	0.003	0.008	0.006
k2	0.028	0.016	0.016	0.009	0.031	0.045
k3	0.110	0.061	0.062	0.047	0.120	0.191
k4	0.002	0.063	0.074	0.041	0.096	0.331
k5	0.352	0.082	0.023	0.008	0.071	0.096
k6	0.002	0.002	0.000	0.000	0.000	0.000
k7	0.004	0.007	0.002	0.004	0.003	0.013
k8	0.000	0.004	0.006	0.000	0.000	0.001
k9	0.009	0.002	0.001	0.002	0.000	0.000
k10	0.001	0.001	0.000	0.000	0.003	0.007
k11	0.005	0.011	0.005	0.004	0.058	0.006

^aDetermined by numerical analysis according to reaction schemes shown in Fig. 7; differential equations used in the numerical analysis are shown in Additional file 3
^bCelEcc fused to the indicated CBM
^cTotal nmol of CelEcc_CBM hybrid added to reaction
^dTotal hexose or pentose monomer added to the reaction as determined from exhaustive hydrolysis of IL-SG
^eFraction of total hexose or pentose present in the reaction converted to soluble products detected by quantitative NIMS analysis

ISSN: 2731-3654