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Table 4 Apparent rates obtained from numerical integration of NIMS time course data

From: Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules

CBM fusionb None CBM3a CBM6 CBM22 CBM30 CBM44
Protein (nmol)c 0.32 0.32 0.32 0.32 0.32 0.32
Total hexose added (mM)d 26.1 26.1 26.1 26.1 26.1 26.1
Fraction hexose reactede 0.33 0.42 0.38 0.29 0.45 0.49
Apparent ratea
 k1 0.013 0.011 0.009 0.009 0.017 0.027
 k2 0.225 0.103 0.111 0.099 0.186 0.232
 k3 0.049 0.024 0.025 0.024 0.035 0.028
 k4 0.000 0.000 0.004 0.001 0.084 0.000
 k5 0.044 0.016 0.007 0.002 0.011 0.003
 k6 0.000 0.009 0.001 0.022 0.016 0.010
 k7 0.000 0.003 0.000 0.008 0.000 0.000
 k8 0.007 0.000 0.003 0.010 0.000 0.011
Total pentose added (mM)d 13.9 13.9 13.9 13.9 13.9 13.9
Fraction pentose reactede 0.56 0.54 0.52 0.50 0.54 0.58
Apparent ratea
 k1 0.006 0.003 0.004 0.003 0.008 0.006
 k2 0.028 0.016 0.016 0.009 0.031 0.045
 k3 0.110 0.061 0.062 0.047 0.120 0.191
 k4 0.002 0.063 0.074 0.041 0.096 0.331
 k5 0.352 0.082 0.023 0.008 0.071 0.096
 k6 0.002 0.002 0.000 0.000 0.000 0.000
 k7 0.004 0.007 0.002 0.004 0.003 0.013
 k8 0.000 0.004 0.006 0.000 0.000 0.001
 k9 0.009 0.002 0.001 0.002 0.000 0.000
 k10 0.001 0.001 0.000 0.000 0.003 0.007
 k11 0.005 0.011 0.005 0.004 0.058 0.006
  1. aDetermined by numerical analysis according to reaction schemes shown in Fig. 7; differential equations used in the numerical analysis are shown in Additional file 3
  2. bCelEcc fused to the indicated CBM
  3. cTotal nmol of CelEcc_CBM hybrid added to reaction
  4. dTotal hexose or pentose monomer added to the reaction as determined from exhaustive hydrolysis of IL-SG
  5. eFraction of total hexose or pentose present in the reaction converted to soluble products detected by quantitative NIMS analysis