Fig. 3From: Leaf-residing Methylobacterium species fix nitrogen and promote biomass and seed production in Jatropha curcas Characterization of nitrogenase. a, b Acetylene reduction activity in vitro. Methylobacterium L2-4 was cultured in N-free medium containing various combination of co-factors (Fe/Mo/V) or concentrations of NH4Cl. a Effects of co-factors. b Sensitivity to ammonium ions. c Nitrogenase activity of in planta. Seedlings of various crops were inoculated with L2-4 strain and AR assays were done 20 days after inoculation. Each value represents mean ± standard deviation (SD) of three replicatesBack to article page