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Table 1 Distribution of nitrogen-fixing and endoglucanase-positive strains originating from different media

From: Leaf-residing Methylobacterium species fix nitrogen and promote biomass and seed production in Jatropha curcas

Media Medium Total number of isolatesa Positive isolates (%)
nifH b ARAc Endoglucanased
Rich media M869 257 132 (51.4) 81 (29.5) 98 (38.1)
  KB 354 206 (58.2) 143 (40.4) 155 (43.8)
Heterotrophic media R2A 189 112 (59.3) 58 (30.7) 75 (39.7)
Minimal media AMS 106 80 (75.5) 49 (46.2) 33 (31.1)
N-free media Nfb 101 68 (67.3) 45 (45.6) 78 (77.2)
  Baz 10 5 (50.0) 5 (50.0) 9 (90.0)
  1. aTotal number of isolates selected for 16S rRNA gene sequencing and phylogeny
  2. bPCR amplification of nifH gene fragments was performed using specific degenerate primers
  3. cAcetylene reduction activity in pure culture was measured by GC
  4. dPlate assays for endoglucanase activity using KM solid medium with 0.2 % CMC were spot inoculated with endophytes and incubated at 30 °C for 3 days