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Table 1 Distribution of nitrogen-fixing and endoglucanase-positive strains originating from different media

From: Leaf-residing Methylobacterium species fix nitrogen and promote biomass and seed production in Jatropha curcas

Media

Medium

Total number of isolatesa

Positive isolates (%)

nifH b

ARAc

Endoglucanased

Rich media

M869

257

132 (51.4)

81 (29.5)

98 (38.1)

 

KB

354

206 (58.2)

143 (40.4)

155 (43.8)

Heterotrophic media

R2A

189

112 (59.3)

58 (30.7)

75 (39.7)

Minimal media

AMS

106

80 (75.5)

49 (46.2)

33 (31.1)

N-free media

Nfb

101

68 (67.3)

45 (45.6)

78 (77.2)

 

Baz

10

5 (50.0)

5 (50.0)

9 (90.0)

  1. aTotal number of isolates selected for 16S rRNA gene sequencing and phylogeny
  2. bPCR amplification of nifH gene fragments was performed using specific degenerate primers
  3. cAcetylene reduction activity in pure culture was measured by GC
  4. dPlate assays for endoglucanase activity using KM solid medium with 0.2 % CMC were spot inoculated with endophytes and incubated at 30 °C for 3 days