Fig. 5
From: Peptide-mediated microalgae harvesting method for efficient biofuel production
Silica-affinity peptide (SAP)-mediated cell harvesting for the F. solaris transformant expressing frustulin1-GFP-(G4S)3-SAP. a Photographs of culture media of the wild-type (left) and transformant displaying GFP-SAP (right) co-incubated with silica particles before and after flocculation. Co-flocculation of diatom transformant cells and silica particles (2.5 g) were observed within 1 min. b Flocculation efficiency of the transformant (blue bars) and wild-type (red bars) with silica particles. Diatom cells were co-cultivated with different concentrations of silica particles in 500 ml cultures. Culture media containing cells and particles were collected at 5 cm below the water surface before and after flocculation, and flocculation efficiency was calculated by Eq. 1. Error bars standard deviation (n = 3)