Fig. 8From: Whole-genome sequence of an evolved Clostridium pasteurianum strain reveals Spo0A deficiency responsible for increased butanol production and superior growthGrowth and metabolite analysis of C. pasteurianum WT, M150B, and ΔSpo0A strains in CGM with 100 g/L crude glycerol. a OD600 shows extended lag phase of WT (blue diamonds) compared to M150B (orange squares) and ΔSpo0A (gray triangles). Red arrows indicate samples taken for analysis. b Metabolite profiles at 40 h (hollow) and 73 h (solid) of WT (blue), M150B (orange), and ΔSpo0A (gray). Error bars represent standard deviation, two biological replicates. Stars indicate t test p value <0.05 between WT and either or both M150B and ΔSpo0A. Note that the initial CGM media contains 1.8 g/L acetic acidBack to article page