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Table 3 Strains and plasmids used in this study

From: Whole-genome sequence of an evolved Clostridium pasteurianum strain reveals Spo0A deficiency responsible for increased butanol production and superior growth

  Description Source
Strain
 Cpa ATCC 6013 Wild-type Clostridium pasteurianum ATCC
 Cpa M150B Mutant isolated from crude glycerol selection This study
Cpa ΔSpo0A Constructed Spo0A deficient strain This study
 NEB Turbo Cloning strain for DNA manipulation NEB
Plasmid
 pCpaDcm2.0 Methylating plasmid DNA 2.0
 pKO-mazF General recombination plasmid Al-Hinai et al. [41]
 pKO-mazF-spo0A Knockout plasmid targeting Spo0A This study
 p94-Flp Flippase plasmid for ThR removal Al-Hinai et al. [41]