Table 3 Strains and plasmids used in this study
| Â | Description | Source |
|---|---|---|
Strain | ||
 Cpa ATCC 6013 | Wild-type Clostridium pasteurianum | ATCC |
 Cpa M150B | Mutant isolated from crude glycerol selection | This study |
Cpa ΔSpo0A | Constructed Spo0A deficient strain | This study |
 NEB Turbo | Cloning strain for DNA manipulation | NEB |
Plasmid | ||
 pCpaDcm2.0 | Methylating plasmid | DNA 2.0 |
 pKO-mazF | General recombination plasmid | Al-Hinai et al. [41] |
 pKO-mazF-spo0A | Knockout plasmid targeting Spo0A | This study |
 p94-Flp | Flippase plasmid for ThR removal | Al-Hinai et al. [41] |