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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824

Fig. 7

Evidence for excision of a prophage from the genome of C. acetobutylicum ATCC strain COSMIC. Screen shot of the mapped paired end reads of ATCC 824 COSMIC against the NC_003030 reference sequence over the region 2016385-2069765 encompassing a prophage. The indicated primers were used to show the region was present both in the chromosome (primers prophage-F1 and prophage-R1 and prophage-F2 and prophage-R2) and as an excised circular form (primers prophage-R1 and prophage-F2). The coverage for the genome was on average just over 200, whereas for the prophage encoding region it was over 4000

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