Table 3 SNVs in the pSOL1 megaplasmid of C. acetobutylicum ATCC 824 COSMIC strain and re-acquired ATCC 824 strain
NC_001988 | Type | Ref | Allele | Feature | Change | AA Change | Function/comment |
|---|---|---|---|---|---|---|---|
ATCC 824 isolate re-acquired from the ATCC in November 2011 | |||||||
 18758 | SNV | G | T | CA_P0017 | 11C > A | Ala4Glu | GrkB germination protein, 4Glu also in SMB_P016 of DSM 1731 |
 54266 | SNV | A | G | CA_P0053 | 317T > C | Leu106Pro | Xylanase XYNB, now identical to SMB_P052 of DSM 1731 |
 56219 | SNV | A | G | CA_P0055 | 506A > G | Asn169Ser | Hypothetical protein, now identical to SMB_P054 of DSM 1731 |
 60555 | SNV | G | T | CA_P0059 | 754C > A | His252Asn | Alcohol dehydrogenase, now identical to SMB_P058 of DSM 1731 |
 191997 | Del | ACTC | – | Deletion | – | – | Same deletion as in pSMBa of DSM1731 |
ATCC 824 COSMIC strain | |||||||
 18758 | SNV | G | T | CA_P0017 | 11C > A | Ala4Glu | GrkB germination protein, 4Glu also in SMB_P016 of DSM 1731 |
 54266 | SNV | A | G | CA_P0053 | 317T > C | Leu106Pro | Xylanase XYNB, now identical to SMB_P052 of DSM 1731 |
 56219 | SNV | A | G | CA_P0055 | 506A > G | Asn169Ser | Hypothetical protein, now identical to SMB_P054 of DSM 1731 |
 60555 | SNV | G | T | CA_P0059 | 754C > A | His252Asn | Alcohol dehydrogenase, now identical to SMB_P058 of DSM 1731 |
 69715 | Del | 69715…74631 | CA_P0066-71 | del4916 bp | – | Unique to pSOL1, affects ptna, manY,ptnd, CA_P0069, CA_P0070, CA_p0071 | |
 191997 | Del | ACTC | – | Deletion | – | – | Same deletion as in pSMBa of DSM1731 |
DSM 1731 (map positions are to NC_015686) | |||||||
 18524 | SNV | C | A | SMB_P016 | 245G > T | Gly82Val | GrkB, germination protein, Gly at position 82 instead of Val in CA_P0017 |
 20626 | SNV | G | A | SMB_P019 | 220C > T | Ala407Val | GrkA, germination receptor, Ala at position 407 instead of Val in CA_P0020 |