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Table 3 SNVs in the pSOL1 megaplasmid of C. acetobutylicum ATCC 824 COSMIC strain and re-acquired ATCC 824 strain

From: Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824

NC_001988 Type Ref Allele Feature Change AA Change Function/comment
ATCC 824 isolate re-acquired from the ATCC in November 2011
 18758 SNV G T CA_P0017 11C > A Ala4Glu GrkB germination protein, 4Glu also in SMB_P016 of DSM 1731
 54266 SNV A G CA_P0053 317T > C Leu106Pro Xylanase XYNB, now identical to SMB_P052 of DSM 1731
 56219 SNV A G CA_P0055 506A > G Asn169Ser Hypothetical protein, now identical to SMB_P054 of DSM 1731
 60555 SNV G T CA_P0059 754C > A His252Asn Alcohol dehydrogenase, now identical to SMB_P058 of DSM 1731
 191997 Del ACTC Deletion Same deletion as in pSMBa of DSM1731
ATCC 824 COSMIC strain
 18758 SNV G T CA_P0017 11C > A Ala4Glu GrkB germination protein, 4Glu also in SMB_P016 of DSM 1731
 54266 SNV A G CA_P0053 317T > C Leu106Pro Xylanase XYNB, now identical to SMB_P052 of DSM 1731
 56219 SNV A G CA_P0055 506A > G Asn169Ser Hypothetical protein, now identical to SMB_P054 of DSM 1731
 60555 SNV G T CA_P0059 754C > A His252Asn Alcohol dehydrogenase, now identical to SMB_P058 of DSM 1731
 69715 Del 69715…74631 CA_P0066-71 del4916 bp Unique to pSOL1, affects ptna, manY,ptnd, CA_P0069, CA_P0070, CA_p0071
 191997 Del ACTC Deletion Same deletion as in pSMBa of DSM1731
DSM 1731 (map positions are to NC_015686)
 18524 SNV C A SMB_P016 245G > T Gly82Val GrkB, germination protein, Gly at position 82 instead of Val in CA_P0017
 20626 SNV G A SMB_P019 220C > T Ala407Val GrkA, germination receptor, Ala at position 407 instead of Val in CA_P0020