Fig. 1From: Engineering transcription factors to improve tolerance against alkane biofuels in Saccharomyces cerevisiae Site mutagenesis of PDR1 and PDR3, and plasmid construction. Plasmid pESC-Ura (http://www.chem-agilent.com) was used as a vector to express the transcription factors. Wild-type and mutant alleles of PDR1 and PDR3 were cloned into MCS2 and MCS1, respectively. Asterisks mutation sitesBack to article page