Fig. 4

Analyses of intracellular alkane levels in BYL13 expressing Pdr transcription factors. a Upon exposure to C10 and C11 alkanes, respectively, intracellular alkanes were extracted, measured by GC, and quantified by normalizing areas of GC peaks to those of an internal standard (IS) n-dodecane as well as corresponding protein amount. b To identify involvement of ABC efflux pumps in reducing intracellular alkanes, intracellular alkanes were compared between BYL13 expressing Pdr transcription factors with and without NaN₃. c To verify lower import of alkanes, intracellular alkanes were compared between BYL13 with pESC-Ura and expressing Pdr transcription factors in the presence of NaN₃. Alkane amount in cells with pESC-Ura, and with NaN₃ were set as 1. Asterisks significant difference (one-tailed Student t test, p < 0.05); error bars SD from three biological replicates