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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Engineering transcription factors to improve tolerance against alkane biofuels in Saccharomyces cerevisiae

Fig. 4

Analyses of intracellular alkane levels in BYL13 expressing Pdr transcription factors. a Upon exposure to C10 and C11 alkanes, respectively, intracellular alkanes were extracted, measured by GC, and quantified by normalizing areas of GC peaks to those of an internal standard (IS) n-dodecane as well as corresponding protein amount. b To identify involvement of ABC efflux pumps in reducing intracellular alkanes, intracellular alkanes were compared between BYL13 expressing Pdr transcription factors with and without NaN3. c To verify lower import of alkanes, intracellular alkanes were compared between BYL13 with pESC-Ura and expressing Pdr transcription factors in the presence of NaN3. Alkane amount in cells with pESC-Ura, and with NaN3 were set as 1. Asterisks significant difference (one-tailed Student t test, p < 0.05); error bars SD from three biological replicates

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