Table 1 Strains, plasmids and PDR alleles used in this study
Strains, plasmids, and genes | Description | Sources |
|---|---|---|
S. cerevisiae strains | ||
 BY4741 | MATa; his3∆1; leu2∆0; met15∆0; ura3∆0 | ATCC201388 |
 BYL1K | pdr1::kanMX derived from BY4741 | This study |
 BYL13K | pdr3::kanMX derived from BYL1K | This study |
 BYL13 | pdr1∆ pdr3∆, KanMX-free BYL13K | This study |
Plasmids | ||
 pESC-Ura | Expression vector, Ura+ | Agilent |
 pRS42K | Expression vector, Kan+ | Euroscarf |
 pSH47 | A plasmid for marker rescue, Ura+ | Euroscarf |
 pUG6 | A plasmid with a KanMX gene cassette, Amp+ | Euroscarf |
PDR1 and PDR3 alleles | ||
 PDR1 wt | Wild-type PDR1 | This study |
 PDR1 mt1 | A site mutant of PDR1 with amino acid substitution of F815S | This study, [24] |
 PDR1 mt2 | A site mutant of PDR1 with amino acid substitution of R821S | This study, [21] |
 PDR1 mt1+2 | A site mutant of PDR1 with amino acid substitution of both F815S and R821S | This study |
 PDR3 wt | Wild-type PDR3 | This study |
 PDR3 mt | A site mutant of PDR3 with amino acid substitution of Y276H | This study, [23] |