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Table 1 Strains, plasmids and PDR alleles used in this study

From: Engineering transcription factors to improve tolerance against alkane biofuels in Saccharomyces cerevisiae

Strains, plasmids, and genes Description Sources
S. cerevisiae strains
 BY4741 MATa; his3∆1; leu2∆0; met15∆0; ura3∆0 ATCC201388
 BYL1K pdr1::kanMX derived from BY4741 This study
 BYL13K pdr3::kanMX derived from BYL1K This study
 BYL13 pdr1∆ pdr3∆, KanMX-free BYL13K This study
Plasmids
 pESC-Ura Expression vector, Ura+ Agilent
 pRS42K Expression vector, Kan+ Euroscarf
 pSH47 A plasmid for marker rescue, Ura+ Euroscarf
 pUG6 A plasmid with a KanMX gene cassette, Amp+ Euroscarf
PDR1 and PDR3 alleles
 PDR1 wt Wild-type PDR1 This study
 PDR1 mt1 A site mutant of PDR1 with amino acid substitution of F815S This study, [24]
 PDR1 mt2 A site mutant of PDR1 with amino acid substitution of R821S This study, [21]
 PDR1 mt1+2 A site mutant of PDR1 with amino acid substitution of both F815S and R821S This study
 PDR3 wt Wild-type PDR3 This study
 PDR3 mt A site mutant of PDR3 with amino acid substitution of Y276H This study, [23]