Skip to main content
Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Overcoming substrate limitations for improved production of ethylene in E. coli

Fig. 1

a Putative metabolic scheme for ethylene production in E. coli via the Ethylene-Forming Enzyme (EFE) and the formation of glutamate from α-ketoglutarate (AKG); genes responsible for the catalytic steps or regulation relevant to this work indicated in red (knockout) and green (overexpression). b Ethylene production, headspace O2, and growth over time in LB media for E. coli (MG1655) harboring the pUC-Plac-efe-Flag plasmid revealing ethylene production to peak at an optical density between 0.2 and 0.3. P5C: L-Δ1-pyrroline-5-carboxylate. Gene abbreviations: icd (isocitrate dehydrogenase), gdhA (glutamate dehydrogenase), gltBD (glutamate synthase), argR (transcriptional regulator of arginine biosynthesis), sucA (2-oxoglutarate decarboxylase)

Back to article page