Table 1 Plasmids and Strains
Plasmids and strains | Description | References |
|---|---|---|
Plasmids | ||
 pRS424 | TRP1, a multicopy plasmid | [76] |
 pRS425 | LEU2, a multicopy plasmid | [76] |
 pRS424-WHI2 | pRS424 with insert of S288c yeast genomic DNA fragment chrXV: 409,259-412,369 (containing complete sequence of the WHI2 gene) | This study |
 pRS424GPD | pRS424 with GPD promoter | [76] |
 pRS425GPD | pRS425 with GPD promoter | [76] |
 pRS424GPD-WHI2 | WHI2 expressed in pRS424GPD | This study |
 pRS424GPD-SFP1 | SFP1 expressed in pRS424GPD | This study |
 pRS424GPD-ACE2 | ACE2 expressed in pRS424GPD | This study |
 pRS425GPD-SFP1 | SFP1 expressed in pRS425GPD | This study |
 pRS425GPD-ACE2 | ACE2 expressed in Prs425GPD | This study |
Strains | ||
 D452-2 | MATa, leu2, his3, ura3, can1 | [77] |
 SR8 | D452-2 expressing XYL1, XYL2, and XKS1 through integration, evolutionary engineering in xylose-containing media, and ALD6 deletion | [40] |
 SR8-trp | SR8 with TRP1 disrupted | Developed in Dr. Yong-Su Jin lab |
 SR8-4 | SR8 with TRP1, LEU2, HIS3 and URA3 disrupted | Developed in Dr. Yong-Su Jin lab |
 S-C1 | SR8-trp harboring pRS424GPD, as a control | This study |
 YC1 | SR8-trp harboring pRS424-WHI2 | This study |
 S- SFP1 | SR8-trp harboring pRS424GPD- SFP1 | This study |
 S-ACE2 | SR8-trp harboring pRS424GPD- ACE2 | This study |
S-C2 | SR8-4 harboring pRS424GPD and pRS425GPD, as a control | This study |
 S-WHI2-c | SR8-4 harboring pRS424GPD-WHI2 and pRS425GPD | This study |
 S-WHI2-SFP1 | SR8-4 harboring pRS424GPD- WHI2 and pRS425GPD- SFP1 | This study |