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Table 1 Plasmids and Strains

From: Transcriptional profiling reveals molecular basis and novel genetic targets for improved resistance to multiple fermentation inhibitors in Saccharomyces cerevisiae

Plasmids and strains Description References
Plasmids
 pRS424 TRP1, a multicopy plasmid [76]
 pRS425 LEU2, a multicopy plasmid [76]
 pRS424-WHI2 pRS424 with insert of S288c yeast genomic DNA fragment chrXV: 409,259-412,369 (containing complete sequence of the WHI2 gene) This study
 pRS424GPD pRS424 with GPD promoter [76]
 pRS425GPD pRS425 with GPD promoter [76]
 pRS424GPD-WHI2 WHI2 expressed in pRS424GPD This study
 pRS424GPD-SFP1 SFP1 expressed in pRS424GPD This study
 pRS424GPD-ACE2 ACE2 expressed in pRS424GPD This study
 pRS425GPD-SFP1 SFP1 expressed in pRS425GPD This study
 pRS425GPD-ACE2 ACE2 expressed in Prs425GPD This study
Strains
 D452-2 MATa, leu2, his3, ura3, can1 [77]
 SR8 D452-2 expressing XYL1, XYL2, and XKS1 through integration, evolutionary engineering in xylose-containing media, and ALD6 deletion [40]
 SR8-trp SR8 with TRP1 disrupted Developed in Dr. Yong-Su Jin lab
 SR8-4 SR8 with TRP1, LEU2, HIS3 and URA3 disrupted Developed in Dr. Yong-Su Jin lab
 S-C1 SR8-trp harboring pRS424GPD, as a control This study
 YC1 SR8-trp harboring pRS424-WHI2 This study
 S- SFP1 SR8-trp harboring pRS424GPD- SFP1 This study
 S-ACE2 SR8-trp harboring pRS424GPD- ACE2 This study
S-C2 SR8-4 harboring pRS424GPD and pRS425GPD, as a control This study
 S-WHI2-c SR8-4 harboring pRS424GPD-WHI2 and pRS425GPD This study
 S-WHI2-SFP1 SR8-4 harboring pRS424GPD- WHI2 and pRS425GPD- SFP1 This study
  1. The TDH3 promoter is often referred to as the GPD promoter, which is used in the pRS4XX series of expression vectors [76]
  2. GPD stands for Glyceraldehyde-3-phosphate dehydrogenase, encoded by the TDH3 gene