Fig. 1

Alignment of protein sequences belonging to glycoside hydrolase family 1 from Trichoderma. Alignment was determined by the MUSCLE multiple sequence alignment program [60]. Conserved amino acids are indicated by a black background. The T. reesei BGLII (V409F) point mutation is indicated by the box at the top. The secondary structures of BGLII are also indicated at the top. The glycone and aglycone binding sites are indicated by open circles and filled diamonds, respectively [37]. The protein sequences obtained from the NCBI database are: T. reesei BGLII (Accession number: BAA74959), T. viride beta-glucosidase 2 (Accession number: AAQ21384), T. longibrachiatum beta-glucosidase II (Accession number: ACZ34300), T. atroviride glycoside hydrolase family 1 protein (Accession number: EHK41167), T. virens glycoside hydrolase family 1 protein (Accession number: EHK16702), T. harzianum 1,4-beta-glucosidase (Accession number: AFD01233), T. harzianum beta-1,4-glucosidase (Accession number: ABN50090), and T. reesei Cel1b (Accession number AAP57758)