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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: The impact of a single-nucleotide mutation of bgl2 on cellulase induction in a Trichoderma reesei mutant

Fig. 3

Chromatogram of transglycosylation products by rBGLIIwt. HPLC analysis of transglycosylation products using purified recombinant wild-type BGLII in E. coli. a The chromatogram generated by the Prominence HPLC system. In the standard chromatogram, G4, G3, G2, and G1 represent cellotetraose, cellotriose, cellobiose, and glucose peaks, respectively. Other β-disaccharides, α-sophorose, laminaribiose, and gentiobiose are represented by Sp, Lm, and Ge, respectively. b The chromatogram of size exclusion chromatography. Cello-oligosaccharides were used as the standard substance. Putative transglycosylation products are indicated by arrows. Asterisk refers to the peak of a buffer component in the reactant

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