Fig. 5
From: Engineering acyl carrier protein to enhance production of shortened fatty acids
Free fatty acid production by C12 thioesterase. a Representative GC-MS trace of FAEEs derived from cell cultures shows thioesterase specificity toward 12-carbon acyl chains. b FFA concentrations measured from cell cultures at 6 h (blue) and 24 h (black) post-induction of both the C12 thioesterase and the indicated ACP. The Se-ACP I75 W and I75Y mutants and their derivatives yield more FFA than controls. Data represent triplicate biological measurements. Error bars are S.E.M