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Table 1 Transformation efficiencies of C. acetobutylicum ATCC824 and MGCΔcac1502 for unmethylated and methylated pCons2.1

From: Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum

  C. acetobutylicum ATCC824 MGCΔcac1502
Unmethylated pCons2.1 0 0.79 (±0.24) × 104
Methylated pCons2.1 0.46 (±0.11) × 104 0.58 (±0.18) × 104
  1. Values are expressed in number of transformants per μg DNA
  2. Mean values and standard deviations from three independent experiments are given
  3. 25 ng pCons2.1 was used in each experiment