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Table 2 Transformation efficiencies of MGCΔcac1502 and MGCΔcac1502ΔuppΔcac3535 for unmethylated pCons2.1

From: Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum

  MGCΔcac1502 MGC Δcac1502Δupp Δcac3535
Unmethylated pCons2.1 0.79 (±0.24) × 104 6.1 (±3.2) × 104
  1. Values are expressed in number of transformants per μg DNA
  2. Mean values and standard deviations from three independent experiments are given
  3. 25 ng pCons2.1 was used in each experiment