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Table 3 Bacterial strains and plasmids used in this study

From: Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum

Strain or plasmid Relevant characteristicsa Source or referenceb
Bacterial strains
 E. coli
  TOP10   Invitrogen
  ER2275 RecA McrBC NEB
 C. acetobutylicum
  ATCC824 Wild type ATCC
  MGCΔcac1502 ΔCA_C1502 This study
  MGCΔcac1502Δupp ΔCA_C 1502Δ CA_C 2879 This study
  MGCΔcac1502ΔuppΔcac3535 ΔCA_C 1502ΔCA_C 2879ΔCA_C 3535 This study
  MGCΔcac1502ΔuppΔcac3535ΔctfAB ΔCA_C 1502ΔCA_C 2879ΔCA_C 3535ΔCA_P0162-3 This study
  MGCΔcac1502ΔuppΔcac3535ΔldhA ΔCA_C 1502ΔCA_C 2879ΔCA_C 3535ΔCA_C 0267 This study
 Plasmids
  pAN1 Cmr, φ3TI, p15A origin [27]
  pKD4 Apr Kmr [18]
  pETSPO Cmr MLSr [4]
  pUC18 Apr Fermentas
  pUC18-FRT-MLS2 Apr MLSr This study
  pCons2-1 Cmr This study
  pCR-BluntII-TOPO Zeor Kmr Invitrogen
  pCIP2-1 Cmr This study
  pREPcac15 Cmr MLSr ΔCA_C1502 This study
  pCIPcac15 Cmr MLSr ΔCA_C1502 This study
  pREPupp Cmr MLSr Δupp This study
  pCP20 Apr Cmr FLP [29]
  pSOS95 Apr MLSr, acetone operon, repL gene, ColE1 origin [32]
  pCLF1 Cmr FLP This study
  pCR4-TOPO-Blunt Apr Kmr Invitrogen
  pCons::upp Cmr MLSr upp This study
  pREPCAC3535::upp Cmr MLSr upp ΔCA_C3535 This study
  pREPctfAB::upp Cmr MLSr upp ΔctfAB This study
  pREPldhA::upp Cmr MLSr upp ΔldhA This study
  1. a RecA homologous recombination abolished, McrBC lacking methylcytosine-specific restriction system, Cm r chloramphenicol resistance, Ap r ampicillin resistance, MLS r macrolide lincosamide and streptogramin B resistance, Zeo r zeomycin resistance, φ3TI φ3TI methyltransferase, repL Gram-positive origin of replication from pIM13
  2. b NEB New England BioLabs, ATCC American Type Culture Collection (Rockville, MD)