Table 4 Oligonucleotides used for PCR amplifications
Primer name | Oligonucleotide sequence |
|---|---|
PKD4.1 | ctggcgccctgagtgcttgcggcagcgtgagggg |
PKD4.2 | agcccggggatctcatgctggagttcttcgccc |
FRT-MLSR-F | tacaggccttgagcgattgtgtaggctggagc |
FRT-MLSR-R | aacaggcctgggatgtaacgcactgagaagccc |
PCONSAccI | ccggggtaccgtcgacctgcagcc |
PCONSEcoRI | gaattccgcgagctcggtacccggc |
ORI3-D | ccatcgatgggggtcatgcatcaatactatcccc |
ORI4-R | gcttccctgttttaatacctttcgg |
FLP1-D | aaaaggatccaaaaggagggattaaaatgccacaatttggtatattatgtaaaacaccacct |
FLP1-R | aaatggcgccgcgtacttatatgcgtctatttatgtaggatgaaaggta |
REP-UPP-F | aaaacagctgggaggaatgaaataatgagtaaagttacac |
REP-UPP-R | aaaacagctgttattttgtaccgaataatctatctccagc |
CAC 1 | aaaggatccatgcacactcataaatttactgtaggaagtctg |
CAC 2 | ggggaggcctaaaaaggggggtcccaaataatatttgccatagtaaccacc |
CAC 3 | ccccctttttaggcctcccctcgaacttattagaatgattaagattccgg |
CAC 4 | aaaggatcctcattaaatttcctccattttaagcctgtc |
CAC 0 | gtgatataattttcctttaaatggaggaggatctg |
CAC 5 | gccgttaatagacattataattccattggc |
CAC-D | gaattcttaaaaatatttggatcattaagcgg |
CAC-R | gttgtattggaatctttgttattatttctccc |
UPP 1 | aaaaggatcctcctgatctattaattcttgatgaaccc |
UPP 2 | ggggaggcctaaaaagggggattgcataaataaaaagggctgaaaaataaatttcag |
UPP 3 | ccccctttttaggcctccccttatttcattcctccattgtattttttttctatttg |
UPP 4 | aaaaggatccgctattatgaataggttaaataagtcagctgg |
UPP 0 | aatacaagcaaagagaataggctatgtgcc |
UPP 5 | aatacaagcaaagagaataggctatgtgcc |
UPP-D | ggcatatgaagtaacaagagaaatgcagc |
UPP-R | ataatctatctccagcatctccaagacc |
RM3535 1 | aaaaggatccgcagctttctggaaggactacggcg |
RM3535 2 | ggggaggcctaaaaagggggcatttacttatggtacggttcacccc |
RM3535 3 | ccccctttttaggcctccccgtctttaaaaagtaatttatcaaaggcatcaaggc |
RM3535 4 | aaaaggatccctaactctctaaacgttacaatagtaatgcgc |
RM3535 0 | cacattgtcatttataaaagtccctaggg |
RM3535 5 | gtagtaattccaacttcaactcttgccac |
RM3535-D | cttagaatagctgatattgcttgcgg |
RM3535-R | agcatctctcttaatgattctccgg |
CTF1 | aaaaggatcccagacactataatagctttaggtggtacccc |
CTF2 | ggggaggcctaaaaagggggattataaaaagtagttgaaatatgaaggtttaaggttg |
CTF3 | ccccctttttaggcctccccatatccaatgaacttagacccatggctg |
CTF4 | aaaaggatccgtgttataatgtaaatataaataaataggactagaggcg |
CTF0 | taccaccttctttcacgcttggctgcgg |
CTF5 | tatttaaagaggcattatcaccagagcg |
LDH1 | aaaaggatccgctttaaaatttggaaagaggaagttgtg |
LDH2 | ggggaggcctaaaaagggggttagaaatctttaaaaatttctctatagagcccatc |
LDH3 | ccccctttttaggcctccccggtaaaagacctaaactccaagggtggaggctaggtc |
LDH4 | aaaaggatcccccattgtggagaatattccaaagaagaaaataattgc |
LDH0 | cagaaggcaagaatgtattaagcggaaatgc |
LDH5 | cttcccattatagctcttattcacattaagc |
Cac3535-d-SpeI | aaaactagtatgaatgatattaaaatagctttgaaaaaattggttgac |
Cac3535-R-BamHI | aaaaggatccctacaaattatatatatctgttaccaatgcctc |