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Table 3 Amounts of protein of desorbed CEL from lignin-CEL complex by washing with fresh buffer

From: Effects of lignin and surfactant on adsorption and hydrolysis of cellulases on cellulose

 

Protein in supernatant (mg/ml)

Percent of total protein (%)

Hydrolysis yieldc (%)

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0.39

100

ndd

Adsorption supernatant

0.17

43.6

5.9

 First supernatanta

0.04

10.3

4.0

 Second supernatant

0.02

5.1

1.6

 Third supernatant

0.02

5.1

1.2

Desorption at 50 °Cb

0.06

15.4

4.0

Desorption at 4 °Cb

0.02

5.1

ndd

  1. aThe first to third supernatant obtained from the solid washed with 2 ml buffer at room temperature
  2. bThe desorption supernatant obtained from the lignin-CEL complex incubated with 2 ml buffer at 50 or 4 °C for 10 min
  3. cThe hydrolysis of 1 % Avicel was performed at 50 °C for 48 h with a working volume of 3 ml
  4. dNot determined
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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