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Table 3 Amounts of protein of desorbed CEL from lignin-CEL complex by washing with fresh buffer

From: Effects of lignin and surfactant on adsorption and hydrolysis of cellulases on cellulose

  Protein in supernatant (mg/ml) Percent of total protein (%) Hydrolysis yieldc (%)
Loading 0.39 100 ndd
Adsorption supernatant 0.17 43.6 5.9
 First supernatanta 0.04 10.3 4.0
 Second supernatant 0.02 5.1 1.6
 Third supernatant 0.02 5.1 1.2
Desorption at 50 °Cb 0.06 15.4 4.0
Desorption at 4 °Cb 0.02 5.1 ndd
  1. aThe first to third supernatant obtained from the solid washed with 2 ml buffer at room temperature
  2. bThe desorption supernatant obtained from the lignin-CEL complex incubated with 2 ml buffer at 50 or 4 °C for 10 min
  3. cThe hydrolysis of 1 % Avicel was performed at 50 °C for 48 h with a working volume of 3 ml
  4. dNot determined