Table 3 Primers, standard curve parameters and number of gene copies ml⁻¹ sample of the investigated genera

F primer

240F (CCTATCAGGTAGTAGTGGGTGTAAT)

210F (TTTCGCCTAAGGATGGGTCT)

298F (GGAGCAAGAGCCCGGAGT)

594F (TAAGTCTTTGGTGAAAGCTT)

R primer

589R (CCCGGAGGACTGACCAAA)

367R (CGATTTCTCACATTGCGGAG)

586R (CCAAGAGACTTAACAACCCA)

747R (GTTACTCACCGTCAAGAT)

R ² value

0.9999

0.9993

0.9999

0.9994

Slope

−4.53

−3.958

−4.679

-4.358

Intersept

38.913

35.106

43.209

38.283

Efficiency

0.66

0.79

0.64

0.70

CS

5.80E+07 (3.24E+06) a

2.99E+07 (3.98E+06) a

1.98E+06 (8.95E+05) a

2.31E+06 (2.90E+05) a

WR37

2.39E+08 (2.98E+07) ab

2.13E+07 (3.29E+06) ab

4.63E+06 (9.85E+05) ab

2.95E+06 (7.85E+05) ab

WR55

2.48E+07 (4.53E+06) b

1.21E+07 (1.90E+06) b

9.51E+06 (1.74E+06) b

7.66E+05 (1.98E+05) b

PR37

2.03E+08 (1.71E+07) a

2.62E+07 (2.55E+06) a

1.04E+07 (1.63E+06) a

2.91E+06 (6.09E+05) a

PR55

4.75E+07 (3.28E+06) b

1.21E+07 (1.28E+06) b

1.95E+07 (6.18E+06) b

7.83E+05 (1.51E+04) b

WR37us+en

1.67E+08 (2.06E+07) b

2.09E+07 (3.56E+06) ab

7.23E+06 (5.46E+05) ab

2.11E+06 (1.23E+05) ab

PR37us+en

1.54E+08 (1.85E+07) ab

3.40E+07 (4.48E+06) a

1.12E+07 (2.98E+06) a

7.15E+05 (9.79E+04) a