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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

Fig. 3

Confirmation of pMTL83253 presence in C. pasteurianum NRRL B-598 erythromycin-resistant transformants. a pMTL83253 isolated from E. coli JM110 (a1) and C. pasteurianum NRRL B-598 transformants (a2). b pMTL83253 isolated from E. coli JM110 (b2) and C. pasteurianum NRRL B-598 transformants (b3) cleaved by PstI (resulting fragments 370 bp and 4413 bp) compared to the GeneRuler 1 kb DNA ladder - Thermo Scientific (b1)

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