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Table 3 Influence of DNA methylation stage to the electrotransformation efficiency

From: Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

DNA amount [μg]/E. coli strain (designation) CFU (average count)a Efficiency (CFU per μg DNA)
2 μg/DH5α (dam +/dcm +) 3 1.5
2 μg/GM33 (dam −/dcm +) 27 13.5
2 μg/BL21 (dam +/dcm −) 28 14
2 μg/JM110 (dam −/dcm −) 236 118
  1. a2 μg of DNA was used for transformation