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Table 3 Influence of DNA methylation stage to the electrotransformation efficiency

From: Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

DNA amount [μg]/E. coli strain (designation)

CFU (average count)a

Efficiency (CFU per μg DNA)

2 μg/DH5α (dam +/dcm +)

3

1.5

2 μg/GM33 (dam −/dcm +)

27

13.5

2 μg/BL21 (dam +/dcm −)

28

14

2 μg/JM110 (dam −/dcm −)

236

118

  1. a2 μg of DNA was used for transformation
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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