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Table 4 Summary of pMTL83353 containing CFU yielded by conjugation, sonoporation, and combined sono/electroporation approaches

From: Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

Method CFU (average count) Efficiency (CFU per μg DNA)
Conjugation (E. coli JM110 containing RP4 and pMTL83253 donor)  
6 h of conjugation 12
24 h of conjugation 37
Sonoporation
10 % PEG 8000 buffer, 20 s pulse 225a 112.5
30 % PEG 8000 buffer, 20 s pulse 321a 160.5
Sono/electroporation
 30 % PEG 8000 buffer, 20 s ultrasound pulse, 5 ms square-wave pulse 5 ms (1250 V) 265b 530
  1. a2 μg of DNA was used for transformation
  2. b0.5 μg of DNA was used for transformation