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Table 5 Summary of bacterial strains and plasmid DNA used in this thesis

From: Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

Bacterial strains Genotype Source
Clostridium pasteurianum NRRL B-598 ARL collection (NRRL)
 Clostridium pasteurianum DSM 525 (ATCC 6013) Coding type II restriction system CpaAI DSMZ
 Escherichia coli DH5α (DSM 6897) dam +/dcm+ DSMZ
 Escherichia coli JM110 (DSM 11539) dam−/dcm DSMZ
 Escherichia coli BL21(DE3) dam+/dcm CGSC
 Escherichia coli GM33 dam−/dcm+ CGSC
 Escherichia coli HB101 (DSM 1607) dam+/dcm+ DSMZ
E. coli plasmids
 RP4 (RK2) Coding IncP-based conjugation function DSMZ
E. coli/Clostridium shuttle plasmids
 pMTL83353 aad9, pCB102 origin of replication [21]
 pMTL82251 ermB, pBP1 origin of replication [21]
 pMTL83253 ermB, pCB102 origin of replication [21]
 pMTL84251 ermB, pCD6 origin of replication [21]
 pMTL85251 ermB, pIM13 origin of replication [21]