Table 5 Summary of bacterial strains and plasmid DNA used in this thesis
Bacterial strains | Genotype | Source |
|---|---|---|
Clostridium pasteurianum NRRL B-598 | ARL collection (NRRL) | |
 Clostridium pasteurianum DSM 525 (ATCC 6013) | Coding type II restriction system CpaAI | DSMZ |
 Escherichia coli DH5α (DSM 6897) | dam +/dcm+ | DSMZ |
 Escherichia coli JM110 (DSM 11539) | dam−/dcm− | DSMZ |
 Escherichia coli BL21(DE3) | dam+/dcm− | CGSC |
 Escherichia coli GM33 | dam−/dcm+ | CGSC |
 Escherichia coli HB101 (DSM 1607) | dam+/dcm+ | DSMZ |
E. coli plasmids | ||
 RP4 (RK2) | Coding IncP-based conjugation function | DSMZ |
E. coli/Clostridium shuttle plasmids | ||
 pMTL83353 | aad9, pCB102 origin of replication | [21] |
 pMTL82251 | ermB, pBP1 origin of replication | [21] |
 pMTL83253 | ermB, pCB102 origin of replication | [21] |
 pMTL84251 | ermB, pCD6 origin of replication | [21] |
 pMTL85251 | ermB, pIM13 origin of replication | [21] |