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Table 5 Summary of bacterial strains and plasmid DNA used in this thesis

From: Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

Bacterial strains

Genotype

Source

Clostridium pasteurianum NRRL B-598

ARL collection (NRRL)

 Clostridium pasteurianum DSM 525 (ATCC 6013)

Coding type II restriction system CpaAI

DSMZ

 Escherichia coli DH5α (DSM 6897)

dam +/dcm+

DSMZ

 Escherichia coli JM110 (DSM 11539)

dam−/dcm

DSMZ

 Escherichia coli BL21(DE3)

dam+/dcm

CGSC

 Escherichia coli GM33

dam−/dcm+

CGSC

 Escherichia coli HB101 (DSM 1607)

dam+/dcm+

DSMZ

E. coli plasmids

 RP4 (RK2)

Coding IncP-based conjugation function

DSMZ

E. coli/Clostridium shuttle plasmids

 pMTL83353

aad9, pCB102 origin of replication

[21]

 pMTL82251

ermB, pBP1 origin of replication

[21]

 pMTL83253

ermB, pCB102 origin of replication

[21]

 pMTL84251

ermB, pCD6 origin of replication

[21]

 pMTL85251

ermB, pIM13 origin of replication

[21]