Acceleration of biodetoxification on dilute acid pretreated lignocellulose feedstock by aeration and the consequent ethanol fermentation evaluation

Table 1 Simultaneous saccharification and ethanol fermentation (SSF) using dry dilute acid pretreated and biodetoxified corn stover feedstock with changing biodetoxification time

Detoxification time (day)	Inhibitors in hydrolysate (g/L)						Sugars in hydrolysate^a (g/L)		Ethanol titer (g/L)	Ethanol productivity (g/L/h)	Ethanol yield^b (%)
Detoxification time (day)	Furfural	HMF	Acetate	Vanillin	4-HBA	Syringaldehyde	Glucose	Xylose	Ethanol titer (g/L)	Ethanol productivity (g/L/h)	Ethanol yield^b (%)
0	0.75	0.53	6.16	0.57	0.06	0.25	41.99	44.65	5.31	0.39	5.97
1	0.35	0.44	5.88	0.45	0.03	0.19	71.57	48.65	46.16	1.27	58.34
2	0.16	0.24	4.99	0.22	0.02	0.06	71.52	45.19	46.85	1.60	57.08
3	0.07	0.20	4.07	0.03	0.02	0.03	74.27	44.24	47.88	2.12	57.28
4	0.00	0.00	1.28	0.09	0.02	0.05	81.13	29.58	50.00	2.72	59.63
5	0.00	0.00	1.37	0.08	0.02	0.05	79.28	28.58	48.19	2.90	55.70
7	0.00	0.00	1.35	0.08	0.02	0.06	82.47	23.07	51.07	2.62	57.29
9	0.00	0.00	0.75	0.02	0.02	0.03	81.25	14.61	50.41	2.36	55.30
12	0.00	0.00	1.63	0.02	0.01	0.03	83.01	10.49	52.33	2.66	57.30
15	0.00	0.00	0.80	0.01	0.01	0.02	91.31	12.10	53.88	2.67	59.38

SSF was initiated by pre-hydrolysis for 12 h and then the adapted S. cerevisiae DQ1 was inoculated into the hydrolysate at 10 % inoculum to start SSF. SSF conditions: 30 % solids loading, 15 FPU/g DM cellulase dosage, 150 rpm agitation rate; Pre-hydrolysis was conducted at 50 °C, pH 4.8, and SSF was conducted at 37 °C, pH 5.5, respectively
^aGlucose and xylose concentration were detected in the hydrolysate after 12 h’ enzymatic hydrolysis by cellulase
^bEthanol yield was calculated using the methods described by Zhang [41]

ISSN: 2731-3654