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Table 1 Simultaneous saccharification and ethanol fermentation (SSF) using dry dilute acid pretreated and biodetoxified corn stover feedstock with changing biodetoxification time

From: Acceleration of biodetoxification on dilute acid pretreated lignocellulose feedstock by aeration and the consequent ethanol fermentation evaluation

Detoxification time (day) Inhibitors in hydrolysate (g/L) Sugars in hydrolysatea (g/L) Ethanol titer (g/L) Ethanol productivity (g/L/h) Ethanol yieldb (%)
Furfural HMF Acetate Vanillin 4-HBA Syringaldehyde Glucose Xylose
0 0.75 0.53 6.16 0.57 0.06 0.25 41.99 44.65 5.31 0.39 5.97
1 0.35 0.44 5.88 0.45 0.03 0.19 71.57 48.65 46.16 1.27 58.34
2 0.16 0.24 4.99 0.22 0.02 0.06 71.52 45.19 46.85 1.60 57.08
3 0.07 0.20 4.07 0.03 0.02 0.03 74.27 44.24 47.88 2.12 57.28
4 0.00 0.00 1.28 0.09 0.02 0.05 81.13 29.58 50.00 2.72 59.63
5 0.00 0.00 1.37 0.08 0.02 0.05 79.28 28.58 48.19 2.90 55.70
7 0.00 0.00 1.35 0.08 0.02 0.06 82.47 23.07 51.07 2.62 57.29
9 0.00 0.00 0.75 0.02 0.02 0.03 81.25 14.61 50.41 2.36 55.30
12 0.00 0.00 1.63 0.02 0.01 0.03 83.01 10.49 52.33 2.66 57.30
15 0.00 0.00 0.80 0.01 0.01 0.02 91.31 12.10 53.88 2.67 59.38
  1. SSF was initiated by pre-hydrolysis for 12 h and then the adapted S. cerevisiae DQ1 was inoculated into the hydrolysate at 10 % inoculum to start SSF. SSF conditions: 30 % solids loading, 15 FPU/g DM cellulase dosage, 150 rpm agitation rate; Pre-hydrolysis was conducted at 50 °C, pH 4.8, and SSF was conducted at 37 °C, pH 5.5, respectively
  2. aGlucose and xylose concentration were detected in the hydrolysate after 12 h’ enzymatic hydrolysis by cellulase
  3. bEthanol yield was calculated using the methods described by Zhang [41]