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Table 1 Time-course GC–MS metabolome analysis of CBP bioconversions; aqueous concentrations (mg/L) and the fold change (low S/G to high S/G) of potential biomass-derived inhibitors

From: Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure

Metabolite 0 h 71 h 140 h
Low S/G High S/G Fold change Low S/G High S/G Fold change Low S/G High S/G Fold change
p-Hydroxybenzoic acid 1.28 0.14 9.33 4.54 0.56 8.08 6.82 1.09 6.26
4-Hydroxyphenethyl alcohol 0.02 0.01 1.50 0.26 0.77 0.34 0.46 1.73 0.27
11.64 347 0.38 0.16 2.37 4.62 0.97 4.78 8.23 1.74 4.72
11.00 218 100 2.13 0.55 3.89 5.16 1.28 4.04 4.20 1.30 3.23
10.92 218 100 277 3.38 1.08 3.14 9.32 3.71 2.51 7.73 2.41 3.21
14.90 Guaiacyl lignan 1.33 0.36 3.73 1.15 0.36 3.19 0.72 0.28 2.61
14.96 Guaiacyl lignan 2.30 0.68 3.36 2.27 0.81 2.80 1.67 0.70 2.37
Guaiacylglycerol 0.09 0.04 2.17 0.10 0.05 2.20 0.09 0.05 2.00
15.52 Syringyl lignan 0.09 0.17 0.55 0.12 0.25 0.49 0.13 0.28 0.46
15.44 Syringyl lignan 0.05 0.10 0.55 0.05 0.10 0.47 0.04 0.10 0.38
Syringylglycerol 0.09 0.09 1.04 0.13 0.12 1.09 0.12 0.13 0.92
  1. Italicized values: higher in the Low S/G poplar bioconversion; Italicized underlined values: higher in the High S/G poplar bioconversion
  2. Only metabolites released with greater than two-fold difference between the low and high S/G phenotypes are shown (with the exception of syringyl glycerol). Numerical labels for unidentified metabolites are denoted by retention time and key mass-to-charge (m/z) ratios