Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure

Table 1 Time-course GC–MS metabolome analysis of CBP bioconversions; aqueous concentrations (mg/L) and the fold change (low S/G to high S/G) of potential biomass-derived inhibitors

Metabolite	0 h			71 h			140 h
Metabolite	Low S/G	High S/G	Fold change	Low S/G	High S/G	Fold change	Low S/G	High S/G	Fold change
p-Hydroxybenzoic acid	1.28	0.14	9.33	4.54	0.56	8.08	6.82	1.09	6.26
4-Hydroxyphenethyl alcohol	0.02	0.01	1.50	0.26	0.77	0.34	0.46	1.73	0.27
11.64 347	0.38	0.16	2.37	4.62	0.97	4.78	8.23	1.74	4.72
11.00 218 100	2.13	0.55	3.89	5.16	1.28	4.04	4.20	1.30	3.23
10.92 218 100 277	3.38	1.08	3.14	9.32	3.71	2.51	7.73	2.41	3.21
14.90 Guaiacyl lignan	1.33	0.36	3.73	1.15	0.36	3.19	0.72	0.28	2.61
14.96 Guaiacyl lignan	2.30	0.68	3.36	2.27	0.81	2.80	1.67	0.70	2.37
Guaiacylglycerol	0.09	0.04	2.17	0.10	0.05	2.20	0.09	0.05	2.00
15.52 Syringyl lignan	0.09	0.17	0.55	0.12	0.25	0.49	0.13	0.28	0.46
15.44 Syringyl lignan	0.05	0.10	0.55	0.05	0.10	0.47	0.04	0.10	0.38
Syringylglycerol	0.09	0.09	1.04	0.13	0.12	1.09	0.12	0.13	0.92

Italicized values: higher in the Low S/G poplar bioconversion; Italicized underlined values: higher in the High S/G poplar bioconversion
Only metabolites released with greater than two-fold difference between the low and high S/G phenotypes are shown (with the exception of syringyl glycerol). Numerical labels for unidentified metabolites are denoted by retention time and key mass-to-charge (m/z) ratios

ISSN: 2731-3654