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Table 1 Comparison of the experimental yields obtained in this study with theoretical yields calculated considering anabolism and catabolism

From: Consistent 1,3-propanediol production from glycerol in mixed culture fermentation over a wide range of pH

 

Theoretical values

Experimental values

Maximal PDO production

Minimal growth yield

Maximal growth yield

Acetate and formate pathway

pH 5

pH 6

pH 7

pH 8

pH 9

Y X/S (g/mol)a

4.45

4.45

7.97

5.72

5.39 ± 0.25

6.18 ± 0.62

5.90 ± 0.14

6.15 ± 0.41

5.87 ± 0.80

Y ATP/S (mol/mol)b

0.44

0.44

0.79

0.57

0.51 ± 0.02

0.59 ± 0.06

0.56 ± 0.01

0.59 ± 0.04

0.56 ± 0.07

Y PDO/S (mol/mol)

0.72

0.50

0.11

0.64

0.61 ± 0.04

0.52 ± 0.01

0.64 ± 0.00

0.63 ± 0.01

0.58 ± 0.01

Y Acetate/S (mol/mol)

0.22

0

0

0.28

0.21 ± 0.02

0.21 ± 0.01

0.27 ± 0.01

0.29 ± 0.02

0.25 ± 0.04

Y Ethanol/S (mol/mol)

0

0

0.79

0

0.07 ± 0.05

0.11 ± 0.07

0

0

0

Y Lactate/S (mol/mol)

0

0.44

0

0

0

0.06 ± 0.01

0.01 ± 0.01

0

0.07 ± 0.00

Y(Formate+H2)/S (mol/mol)

0

0

0.79

0.28

0.06 ± 0.01

0.07 ± 0.00

0.01 ± 0.01

0.17 ± 0.01

0.26 ± 0.02

  1. aThe biomass yield was calculated assuming an elemental composition of C4H7O2N [14] and that all the ATP produced was used for biomass production
  2. bThe ATP yield was calculated from the metabolites measured after 3 day of fermentation: Y ATP/Acetate = 2; Y ATP/Ethanol = 1; Y ATP/Lactate = 1; Y ATP/PDO = 0