Skip to main content

Advertisement

Table 1 Shifting the metabolism of L. lactis from homo-lactic to homo-ethanol

From: A novel cell factory for efficient production of ethanol from dairy waste

Strains Description Growth conditions Carbon balance (%) Lactose Acetate Formate Acetoin 2,3-Butanediol Ethanol
mol/mol glucose
MG1363 Wide type Anaerobic 90 1.8 ± 0.1 ND ND ND ND ND
CS4099 MG1363 Δ3 ldh,ldh, ΔldhB and ΔldhX) Anaerobic 85 ND 0.61 ± 0.02 1.58 ± 0.07 0.08 ± 0.01 0.09 ± 0.01 0.65 ± 0.02
CS4234 MG1363 Δ3 ldh, Δpta Anaerobic 88 ND ND 0.82 ± 0.05 0.35 ± 0.02 0.16 ± 0.03 0.76 ± 0.04
CS4363 MG1363 Δ3 ldh, Δpta, ΔadhE Aerobic 85 ND ND ND 0.85 ± 0.05 ND ND
CS4435 MG1363 Δ3 ldh, Δpta, ΔadhE pCS4268 Anaerobic 87 ND ND ND ND ND 1.75 ± 0.06
  1. Strains were cultivated in defined SA medium with glucose and samples were collected after 24 h. CO2 is included for calculating the carbon balance. Values are averages of three independent experiments and standard deviations are indicated
  2. ldh (ldhB, ldhX) lactate dehydrogenase, pta phosphotransacetylase, adhE alcohol dehydrogenase, pCS4268 pTD6-pdc-adhB (pdc, pyruvate decarboxylase from Zymomonas mobilis, adhB ethanol dehydrogenase from Z. mobilis)