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Table 1 Shifting the metabolism of L. lactis from homo-lactic to homo-ethanol

From: A novel cell factory for efficient production of ethanol from dairy waste

Strains

Description

Growth conditions

Carbon balance (%)

Lactose

Acetate

Formate

Acetoin

2,3-Butanediol

Ethanol

mol/mol glucose

MG1363

Wide type

Anaerobic

90

1.8 ± 0.1

ND

ND

ND

ND

ND

CS4099

MG1363 Δ3 ldh,ldh, ΔldhB and ΔldhX)

Anaerobic

85

ND

0.61 ± 0.02

1.58 ± 0.07

0.08 ± 0.01

0.09 ± 0.01

0.65 ± 0.02

CS4234

MG1363 Δ3 ldh, Δpta

Anaerobic

88

ND

ND

0.82 ± 0.05

0.35 ± 0.02

0.16 ± 0.03

0.76 ± 0.04

CS4363

MG1363 Δ3 ldh, Δpta, ΔadhE

Aerobic

85

ND

ND

ND

0.85 ± 0.05

ND

ND

CS4435

MG1363 Δ3 ldh, Δpta, ΔadhE pCS4268

Anaerobic

87

ND

ND

ND

ND

ND

1.75 ± 0.06

  1. Strains were cultivated in defined SA medium with glucose and samples were collected after 24 h. CO2 is included for calculating the carbon balance. Values are averages of three independent experiments and standard deviations are indicated
  2. ldh (ldhB, ldhX) lactate dehydrogenase, pta phosphotransacetylase, adhE alcohol dehydrogenase, pCS4268 pTD6-pdc-adhB (pdc, pyruvate decarboxylase from Zymomonas mobilis, adhB ethanol dehydrogenase from Z. mobilis)