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Table 2 Optimization of lactose concentrations in different types of media

From: A novel cell factory for efficient production of ethanol from dairy waste

Lactose (g/L) Media types OD600 Ethanol (g/L) Yield (g ethanol/g lactose) Conversion efficiencya
7.2 SAb 2.0 3.2 0.45 0.83
15.1 SAb 2.3 6.7 0.44 0.82
24.0 SAb 2.9 10.3 0.43 0.80
31.8 SAb 3.6 12.0 0.38 0.71
32.0 RWP+YEc 7.2 13.4 0.42 0.78
40.0 RWP+CSLHd 4.0 17.5 0.44 0.82
80.0 RWP+CSLHe 6.0 30.6 0.40 0.71
80.0g RWP+CSLHf 6.0 41.0 0.38 0.70
  1. aConversion efficiency was calculated based on the theoretical maximal yield (0.538 ethanol/g lactose)
  2. bThe composition of SA medium can be seen in reference 20. It includes 19 amino acids, vitamins, and salts. Glucose was replaced by lactose
  3. cDiluted RWP (residual whey permeate) and 0.5 % (w/v) YE (yeast extract)
  4. d–fDiluted RWP (residual whey permeate) and 2.5 % (w/v) CSLH (corn steep liquor hydrolysate), prepared by condition H1
  5. gFed-batch was performed with initial 80 g/L lactose and the details can be found in Fig. 7