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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities

Fig. 2

Overexpression and purification of recombinant Umcel9y-1 in Escherichia coli BL21(DE3). a SDS-PAGE analysis showing the Umcel9y-1 overexpression in Escherichia coli BL21(DE3). M, Markers; (1) Control without IPTG at 28 °C (supernatant); (2) Control without IPTG at 28 °C (precipitant); (3) 15 °C overnight (supernatant); (4) 15 °C overnight (precipitant); (5) 22 °C overnight (supernatant); (6) 22 °C overnight (precipitant); (7) 28 °C overnight (supernatant); (8) 28 °C overnight (precipitant). b SDS-PAGE analysis showing the purification of recombinant Umcel9y-1 by affinity chromatography. M, Marker; (1) Precipitant before loading; (2) Precipitant flow through; (3) Whole cell disruption before loading; (4) Washing through with 10 mM imidazole; (5) Washing through with 20 mM imidazole; (6) Washing through with 50 mM imidazole; (7–8) Purified eluate with 500 mM imidazole. c SDS-PAGE analysis showing the apparent Mw of purified recombinant Umcel9y-1 protein. M, Marker; (1–2) De-His-tag Umcel9y-1; 3, Fusion Umcel9y-1 with His-tag; 4, Repurified de-His-tag Umcel9y-1

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