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Table 1 Steady-state kinetic constants of the recombinant Umcel9y-1 for various substrates

From: A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities

Substrate Glycosyl Linkage K m (mM) K cat (s−1) K cat/K m (s−1 mM−1) V max (μmolmin−1 mg−1)
HEC β (1,4) 28.264 ± 1.649 185.650 ± 5.085 6.569 158.730 ± 5.591
CMC β (1,4) 20.854 ± 1.198 49.212 ± 1.170 2.360 42.191 ± 2.180
Laminarin β (1,3)/β (1,6) 47.300 ± 3.073 127.510 ± 3.842 2.696 109.892 ± 4.351
Barley glucan β (1,3)/β (1,4)/β (1,6) 13.313 ± 0.782 3276.381 ± 184.275 246.104 2808.303 ± 104.388
pNPCel β-Cellobiose 1.381 ± 0.259 466.664 ± 26.168 337.915 400.003 ± 15.661
Cellotriose β (1,4) 151.673 ± 13.005 897.444 ± 46.260 5.917 769.231 ± 22.347
Cellotetraose β (1,4) 18.300 ± 1.680 159.820 ± 9.084 8.733 136.990 ± 6.877
Cellopentaose β (1,4) 0.615 ± 0.097 42.273 ± 4.442 68.732 36.230 ± 3.222
Cellohexose β (1,4) 5.106 ± 0.888 41.670 ± 3.982 8.161 35.711 ± 4.670