Table 1 Steady-state kinetic constants of the recombinant Umcel9y-1 for various substrates
From: A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities
Substrate | Glycosyl Linkage | K m (mM) | K cat (s−1) | K cat/K m (s−1 mM−1) | V max (μmolmin−1 mg−1) |
|---|---|---|---|---|---|
HEC | β (1,4) | 28.264 ± 1.649 | 185.650 ± 5.085 | 6.569 | 158.730 ± 5.591 |
CMC | β (1,4) | 20.854 ± 1.198 | 49.212 ± 1.170 | 2.360 | 42.191 ± 2.180 |
Laminarin | β (1,3)/β (1,6) | 47.300 ± 3.073 | 127.510 ± 3.842 | 2.696 | 109.892 ± 4.351 |
Barley glucan | β (1,3)/β (1,4)/β (1,6) | 13.313 ± 0.782 | 3276.381 ± 184.275 | 246.104 | 2808.303 ± 104.388 |
pNPCel | β-Cellobiose | 1.381 ± 0.259 | 466.664 ± 26.168 | 337.915 | 400.003 ± 15.661 |
Cellotriose | β (1,4) | 151.673 ± 13.005 | 897.444 ± 46.260 | 5.917 | 769.231 ± 22.347 |
Cellotetraose | β (1,4) | 18.300 ± 1.680 | 159.820 ± 9.084 | 8.733 | 136.990 ± 6.877 |
Cellopentaose | β (1,4) | 0.615 ± 0.097 | 42.273 ± 4.442 | 68.732 | 36.230 ± 3.222 |
Cellohexose | β (1,4) | 5.106 ± 0.888 | 41.670 ± 3.982 | 8.161 | 35.711 ± 4.670 |