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Table 2 The effects of various monosaccharides on Umcel9y-1 activity toward barley glucan and cellopentaose

From: A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities

Additive

Concentration

K m (mM)

K cat (s−1)

K cat/K m (s−1 mM−1)

V max (μmolmin−1 mg−1)

Barley glucan

 (No additive)

13.700 ± 0.805

3276.382 ± 184.281

239.152

2808.301 ± 104.394

 D-galactose

100 mM

5.654 ± 0.995

972.222 ± 68.328

171.953

833.333 ± 35.970

 D-arabinose

100 mM

4.404 ± 0.339

972.222 ± 47.514

220.758

833.333 ± 52.142

 D-mannitose

100 mM

5.378 ± 0.826

897.442 ± 77.300

166.872

769.233 ± 44.361

 D-Xylose

100 mM

3. 706 ± 0.360

972.221 ± 43.778

262.337

833.333 ± 63.100

 D-glucose

100 mM

14.039 ± 2.287

1458.334 ± 88.201

103.877

1250.000 ± 59.374

Cellopentaose

 (No additive)

0.615 ± 0.097

42.271 ± 4.436

68.732

36.233 ± 3.223

 D-galactose

200 mM

0.483 ± 0.060

35.572 ± 4.172

73.644

30.492 ± 2.222

1000 mM

0.253 ± 0.024

47.810 ± 6.410

188.972

40.980 ± 5.271

 D-arabinose

200 mM

1.122 ± 0.133

56.910 ± 6.472

50.722

48.783 ± 4.801

1000 mM

0.917 ± 0.097

77.783 ± 6.851

84.820

66.672 ± 6.052

 D-mannitose

200 mM

0.205 ± 0.036

30.461 ± 3.420

148.585

26.112 ± 2.293

1000 mM

0.169 ± 0.048

45.571 ± 5.453

269.645

39.064 ± 4.213

 D-Xylose

200 mM

0.446 ± 0.060

45.570 ± 4.203

102.175

39.064 ± 3.900

1000 mM

0.109 ± 0.012

70.710 ± 6.884

648.716

60.612 ± 6.190