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Table 2 The effects of various monosaccharides on Umcel9y-1 activity toward barley glucan and cellopentaose

From: A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities

Additive Concentration K m (mM) K cat (s−1) K cat/K m (s−1 mM−1) V max (μmolmin−1 mg−1)
Barley glucan
 (No additive) 13.700 ± 0.805 3276.382 ± 184.281 239.152 2808.301 ± 104.394
 D-galactose 100 mM 5.654 ± 0.995 972.222 ± 68.328 171.953 833.333 ± 35.970
 D-arabinose 100 mM 4.404 ± 0.339 972.222 ± 47.514 220.758 833.333 ± 52.142
 D-mannitose 100 mM 5.378 ± 0.826 897.442 ± 77.300 166.872 769.233 ± 44.361
 D-Xylose 100 mM 3. 706 ± 0.360 972.221 ± 43.778 262.337 833.333 ± 63.100
 D-glucose 100 mM 14.039 ± 2.287 1458.334 ± 88.201 103.877 1250.000 ± 59.374
Cellopentaose
 (No additive) 0.615 ± 0.097 42.271 ± 4.436 68.732 36.233 ± 3.223
 D-galactose 200 mM 0.483 ± 0.060 35.572 ± 4.172 73.644 30.492 ± 2.222
1000 mM 0.253 ± 0.024 47.810 ± 6.410 188.972 40.980 ± 5.271
 D-arabinose 200 mM 1.122 ± 0.133 56.910 ± 6.472 50.722 48.783 ± 4.801
1000 mM 0.917 ± 0.097 77.783 ± 6.851 84.820 66.672 ± 6.052
 D-mannitose 200 mM 0.205 ± 0.036 30.461 ± 3.420 148.585 26.112 ± 2.293
1000 mM 0.169 ± 0.048 45.571 ± 5.453 269.645 39.064 ± 4.213
 D-Xylose 200 mM 0.446 ± 0.060 45.570 ± 4.203 102.175 39.064 ± 3.900
1000 mM 0.109 ± 0.012 70.710 ± 6.884 648.716 60.612 ± 6.190