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Table 2 Primers used in quantitative, touch down and standard PCR targeting the fhs gene

From: Bacterial community composition and fhs profiles of low- and high-ammonia biogas digesters reveal novel syntrophic acetate-oxidising bacteria

Primer name

Nucleotide sequence (5′–3)

Annealing temperature (°C)

OTU3 fw

OTU3 rev

TTTGAACCAGCTGGGGAAA

TTAGCTCGCATTGTTGTCG

63

OTU4 fw

OTU4 rev

GTTGAGGAAAATCGGRAAA

CTATCCAATATTATCATTG

55

OTU5 fw

OTU5 rev

CATACGTGAGCCTTCCCTGG

CCGCAAACGATATTCCGCAG

63

OTU6 fw

OTU6 rev

ATTACATAGCTTAGGCAAA

CCACCACAATATTGCCAAG

53

OTU7 fw

AGAGAACCATCTTTAGGGCCA

63

OTU7 rev

TGGTTTTCCACCAAGACCCA

63

OTU8 fw

OTU8 rev

GAGAATTGGCAAGAAGGCCA

CTGCCTCCCAGACCAACTAC

63

OTU9 fw

OTU9 rev

CCTGGGCAAGCTGGACAAG

GTGGGCAAAATCTTGGTTG

63

OTU10 fw

OTU10 rev

TTTGCGAAAATTAAATAAG

ATTAACCGGATTGTCATTG

55