Fig. 1

Purification and substrate specificity of WT. a SDS-PAGE and immunoblot of wild-type Smlt2602. Lane L: Precision Blue plus protein marker (Bio-Rad). Lane 1: SDS-PAGE of purified wild-type Smlt2602. Lane 2: Anti-His₆ immunoblot of purified wild-type Smlt2602. b Specific activity of WT against 1 mg/mL of the indicated substrate in 20 mM sodium phosphate buffer, pH 8.5. Enzymatic activity was monitored by change in absorbance at 235 nm. All reactions were performed in triplicate and error is reported as standard deviation