Fig. 4From: Engineering broad-spectrum digestion of polyuronides from an exolytic polysaccharide lyaseEnhanced poly-GlcA activity via mutagenesis of His208 residue. Specific activity of WT and H208A, H208W, H208F mutants against 1 mg/mL poly-GlcA in 20 mM sodium phosphate buffer, pH 8. Enzymatic activity was monitored by change in absorbance at 235 nm. All reactions were performed in triplicate and error is reported as standard deviationBack to article page