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Table 2 Substrate specificity ratios of mutant lyase

From: Engineering broad-spectrum digestion of polyuronides from an exolytic polysaccharide lyase

 

ManA/GulA

ManA/MG

WT

3.5 ± 0.1

2.3 ± 0.1

R143L

2.6 ± 0.1

1.5 ± 0.1

Q153A

15.1 ± 1.5

6.2 ± 0.3

Q153N

3.9 ± 0.1

3.0 ± 0.3

L155A

21.1 ± 0.6

6.7 ± 0.1

L155N

12.1 ± 0.8

3.9 ± 0.1

N201L

6.6 ± 0.6

3.6 ± 0.1

H206F

2.6 ± 0.1

2.0 ± 0.1

Y258F

3.9 ± 0.2

2.7 ± 0.2

Y263F

15.7 ± 1.7

6.2 ± 0.6

  1. Ratios were calculated by dividing the poly-ManA activity of each mutant over the poly-GulA or poly-MG activity
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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