Skip to main content

Table 2 Substrate specificity ratios of mutant lyase

From: Engineering broad-spectrum digestion of polyuronides from an exolytic polysaccharide lyase

  ManA/GulA ManA/MG
WT 3.5 ± 0.1 2.3 ± 0.1
R143L 2.6 ± 0.1 1.5 ± 0.1
Q153A 15.1 ± 1.5 6.2 ± 0.3
Q153N 3.9 ± 0.1 3.0 ± 0.3
L155A 21.1 ± 0.6 6.7 ± 0.1
L155N 12.1 ± 0.8 3.9 ± 0.1
N201L 6.6 ± 0.6 3.6 ± 0.1
H206F 2.6 ± 0.1 2.0 ± 0.1
Y258F 3.9 ± 0.2 2.7 ± 0.2
Y263F 15.7 ± 1.7 6.2 ± 0.6
  1. Ratios were calculated by dividing the poly-ManA activity of each mutant over the poly-GulA or poly-MG activity