Fig. 1

Metabolic pathway for n-butanol production via reverse β-oxidation in yeast. n-Butanol production and reverse β-oxidation are shown as a process of one or several rotations, respectively, of an acetoacetyl-CoA-derived synthesis pathway. This pathway can generate various compounds with different chain lengths. The different isoenzymes used in this study (Fig. 2, Table 2) are shown for each reaction. The reaction of ^Ss nphT7 (use of malonyl-CoA and acetyl-CoA for production of acetoacetyl-CoA) is shown in light blue