Fig. 5

Comparison of n-butanol production with different strains. n-Butanol concentrations after 74 h of semi-anaerobic fermentations in SMD medium are shown. Compared are the fermentations of the wildtype CEN.PK113-5D, VSY0 (Δadh1-5), and VSY8 (Δadh1-5 sfa1 with adhE ^A267T/E568K) with plasmid pVS6 (n-butanol pathway), with or without coaA overexpression (pVS5_4), and strain VSY10 (Δadh1-6 sfa1 gpd2, with n-butanol pathway genes of pVS6, coaA, and adhE ^A267T/E568K), in the absence or presence of additional 25 µM pantothenate, in SMD medium. Error bars represent the standard deviation of three independent replicates and a statistical analysis is shown in Additional file 2: Table S4