Table 1 Yeast strains and plasmids used in this study

Strains

 CEN.PK113-5D

MATa ura3-52 MAL2-8 c SUC2

EUROSCARF, Frankfurt

 VSY0

MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2 adh1::loxP adh3::loxP adh5::loxP adh4Δ::loxP adh2Δ::LEU2

This work

 VSY8

MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2 adh1::loxP adh3::loxP adh5::loxP adh4Δ::loxP adh2Δ::LEU2 sfa1Δ: ^Ec adhE ^A267T/E568K , hphNT1

This work

 VSY9

MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2 adh1::loxP adh3::loxP adh5::loxP adh4Δ::loxP adh2Δ::LEU2 sfa1Δ: ^Ec adhE ^A267T/E568K , hphNT1 adh6Δ: ^Ec coaA, natNT2

This work

 VSY10

MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2 adh1::loxP adh3::loxP adh5::loxP adh4Δ::loxP adh2Δ::LEU2 sfa1Δ: ^Ec adhE ^A267T/E568K , hphNT1 adh6Δ: ^Ec coaA, natNT2

gpd2Δ: ^Sc ERG10, ^Ca hbd, ^Ca crt, ^Td ter, ^Ca adhE2, ^Ec eutE, kanMX

This work

Plasmids

 pRS41K

CEN6ARS4, kanMX, Amp ^r

[43]

 pRS62K

2µ, kanMX, Amp ^r , shortened HXT7 promoter and CYC1 terminator

[44]

 pRS62N

2µ, natNT2, Amp ^r , shortened HXT7 promoter and CYC1 terminator

[44]

 pVS1_high copy

2µ, kanMX, Amp ^r, reverse β-oxidation: ^Sc ERG10, ^Ca hbd, ^Ca crt, ^Eg ter, ^Ec adhE2

This work

 pVS1

CEN6ARS4, kanMX, Amp ^r, reverse β-oxidation: ^Sc ERG10, ^Ca hbd, ^Ca crt, ^Eg ter, ^Ca adhE2

This work

 pVS4

CEN6ARS4, hphNT1, Amp^r, ^Ec adhE ^A267T/E568K

This work

 pVS6_high copy

2µ, kanMX, Amp ^r, reverse β-oxidation: ^Sc ERG10, ^Ca hbd, ^Ca crt, ^Td ter, ^Ec eutE, ^Ca adhE2

This work

 pVS6

CEN6ARS4, kanMX, Amp ^r, reverse β-oxidation: ^Sc ERG10, ^Ca hbd, ^Ca crt, ^Td ter, ^Ec eutE, ^Ca adhE2

This work

 pVS7_high copy

2µ, kanMX, Amp ^r, reverse β-oxidation: ERG10, ^Ca hbd, ^Ca crt, ^Td ter, ^Ec eutE, ^Ca bdhB

This work

 pVS7

CEN6ARS4, kanMX, Amp ^r, reverse β-oxidation: ^Sc ERG10, ^Ca hbd, ^Ca crt, ^Td ter, ^Ec eutE, ^Ca bdhB

This work

 pVS8_high copy

2µ, kanMX, Amp ^r, reverse β-oxidation: ^Ca thlA, ^Ca hbd, ^Ca crt, ^Td ter, ^Ec eutE, ^Ca bdhB

This work

 pVS8

CEN6ARS4, kanMX, Amp ^r, reverse β-oxidation: ^Ca thlA, ^Ca hbd, ^Ca crt, ^Td ter, ^Ec eutE, ^Ca bdhB

This work

 pVS9

CEN6ARS4, kanMX, Amp ^r, reverse β-oxidation: ^Ss nphT7, ^Ca hbd, ^Ca crt, ^Td ter, ^Ec eutE, ^Ca adhE2

This work

 pVS10

CEN6ARS4, kanMX, Amp ^r, reverse β-oxidation: ^Ss nphT7, ^Ca hbd, ^Ca crt, ^Sco ccr, ^Ec eutE, ^Ca adhE2

This work

 pVS11

CEN6ARS4, kanMX, Amp ^r, reverse β-oxidation: ^Ca thlA, ^Ca hbd, ^Ca crt, ^Td ter, ^Ec eutE, ^Ca adhE2

This work

 pVS5_1

2µ, natNT2, Amp^r, CoA increase: ^Sc FEN2, pMET25-^Ec coaA

This work

 pVS5_2

2µ, natNT2, Amp^r, CoA increase: ^Sc FEN2, ^Sc CAB2

This work

 pVS5_3

2µ, natNT2, Amp^r, CoA increase: ^Sc FEN2

This work

 pVS5_4

2µ, natNT2, Amp^r, CoA increase: pMET25-^Ec coaA

This work

 pVS5_5

2µ, natNT2, Amp^r, CoA increase: ^Ec coaA

This work

 pVS5_6

2µ, natNT2, Amp^r, CoA increase: pMET25-^Sc FEN2

This work