Skip to main content

Table 1 Yeast strains and plasmids used in this study

From: n-Butanol production in Saccharomyces cerevisiae is limited by the availability of coenzyme A and cytosolic acetyl-CoA

Strain or plasmid Characteristics Reference
Strains
 CEN.PK113-5D MATa ura3-52 MAL2-8 c SUC2 EUROSCARF, Frankfurt
 VSY0 MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2 adh1::loxP adh3::loxP adh5::loxP adh4Δ::loxP adh2Δ::LEU2 This work
 VSY8 MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2 adh1::loxP adh3::loxP adh5::loxP adh4Δ::loxP adh2Δ::LEU2 sfa1Δ: Ec adhE A267T/E568K , hphNT1 This work
 VSY9 MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2 adh1::loxP adh3::loxP adh5::loxP adh4Δ::loxP adh2Δ::LEU2 sfa1Δ: Ec adhE A267T/E568K , hphNT1 adh6Δ: Ec coaA, natNT2 This work
 VSY10 MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2 adh1::loxP adh3::loxP adh5::loxP adh4Δ::loxP adh2Δ::LEU2 sfa1Δ: Ec adhE A267T/E568K , hphNT1 adh6Δ: Ec coaA, natNT2
gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX
This work
Plasmids
 pRS41K CEN6ARS4, kanMX, Amp r [43]
 pRS62K , kanMX, Amp r , shortened HXT7 promoter and CYC1 terminator [44]
 pRS62N 2µ, natNT2, Amp r , shortened HXT7 promoter and CYC1 terminator [44]
 pVS1_high copy , kanMX, Amp r, reverse β-oxidation: Sc ERG10, Ca hbd, Ca crt, Eg ter, Ec adhE2 This work
 pVS1 CEN6ARS4, kanMX, Amp r, reverse β-oxidation: Sc ERG10, Ca hbd, Ca crt, Eg ter, Ca adhE2 This work
 pVS4 CEN6ARS4, hphNT1, Ampr, Ec adhE A267T/E568K This work
 pVS6_high copy 2µ, kanMX, Amp r, reverse β-oxidation: Sc ERG10, Ca hbd, Ca crt, Td ter, Ec eutE, Ca adhE2 This work
 pVS6 CEN6ARS4, kanMX, Amp r, reverse β-oxidation: Sc ERG10, Ca hbd, Ca crt, Td ter, Ec eutE, Ca adhE2 This work
 pVS7_high copy 2µ, kanMX, Amp r, reverse β-oxidation: ERG10, Ca hbd, Ca crt, Td ter, Ec eutE, Ca bdhB This work
 pVS7 CEN6ARS4, kanMX, Amp r, reverse β-oxidation: Sc ERG10, Ca hbd, Ca crt, Td ter, Ec eutE, Ca bdhB This work
 pVS8_high copy 2µ, kanMX, Amp r, reverse β-oxidation: Ca thlA, Ca hbd, Ca crt, Td ter, Ec eutE, Ca bdhB This work
 pVS8 CEN6ARS4, kanMX, Amp r, reverse β-oxidation: Ca thlA, Ca hbd, Ca crt, Td ter, Ec eutE, Ca bdhB This work
 pVS9 CEN6ARS4, kanMX, Amp r, reverse β-oxidation: Ss nphT7, Ca hbd, Ca crt, Td ter, Ec eutE, Ca adhE2 This work
 pVS10 CEN6ARS4, kanMX, Amp r, reverse β-oxidation: Ss nphT7, Ca hbd, Ca crt, Sco ccr, Ec eutE, Ca adhE2 This work
 pVS11 CEN6ARS4, kanMX, Amp r, reverse β-oxidation: Ca thlA, Ca hbd, Ca crt, Td ter, Ec eutE, Ca adhE2 This work
 pVS5_1 2µ, natNT2, Ampr, CoA increase: Sc FEN2, pMET25-Ec coaA This work
 pVS5_2 2µ, natNT2, Ampr, CoA increase: Sc FEN2, Sc CAB2 This work
 pVS5_3 2µ, natNT2, Ampr, CoA increase: Sc FEN2 This work
 pVS5_4 2µ, natNT2, Ampr, CoA increase: pMET25-Ec coaA This work
 pVS5_5 2µ, natNT2, Ampr, CoA increase: Ec coaA This work
 pVS5_6 2µ, natNT2, Ampr, CoA increase: pMET25-Sc FEN2 This work
  1. Genes from Saccharomyces cerevisiae (Sc), Clostridium acetobutylicum (Ca), E.coli (Ec), Euglena gracilis (Eg), Streptomyces collinus (Sco), Streptomyces sp. CL190 (Ss), and Treponema denticola (Td) are indicated by prefixes in superscript. The MET25 promoter is indicated as “pMET25”; other promoters are indicated in Additional file 2: Table S1
  2. kanMX G418 resistance, hphNT1 hygromycin resistance, natNT2 nourseothricin resistance, Amp r ampicillin resistance