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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Cellular automata modeling depicts degradation of cellulosic material by a cellulase system with single-molecule resolution

Fig. 5

Degradation of amorphous cellulose by EG and CBH II. Different combinations of k cat for EG and CBH II satisfy the criterion of the experimental V z in amorphous cellulose, but only the combination k cat (EG) > k cat (CBH II) results in a plausible system behavior. a The value of k off affects V z strongly when k cat (EG) < k cat (CBH II), whereas it does not affect V z when k cat (EG) > k cat (CBH II). b Apparent inactivation of CBH II occurs as result of switch of the enzyme into a resting state on collision with structural obstacles or other enzymes. Green lines indicate the fraction of active CBH II enzymes, while red lines indicate the fraction of resting CBH II enzymes. Solid lines met the criteria k cat (EG) < k cat (CBH II) and dotted lines met the criteria k cat (EG) > k cat (CBH II). The k off for CBH II was fixed at 0.7 × 10−3·s−1. A relatively low k off was chosen because the shown effect is particularly pronounced under these conditions (panel a)

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